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目的:对食管贲门双源癌和单发食管癌及单发贲门癌患者血清蛋白质组和癌组织基因变化差异进行分析,筛选食管癌、贲门癌关键候选蛋白和基因。方法:采用表面增强激光解析离子化-飞行时间-质谱(SELDI-TOF-MS)与比较基因组杂交(CGH)技术分析比较食管贲门双源癌(4例)和单发食管癌(107例)、单发贲门癌(86例)患者血清蛋白质组和癌组织全基因组。结果:根据SELDI-TOF-MS蛋白质组所筛选的候选蛋白与CGH所筛选的基因进行一致性对比分析发现:双源癌中食管癌血清蛋白质组和癌组织CGH变异基因位点相一致的蛋白有14种(52%,11/21);双源癌中贲门癌与单发贲门癌位点相一致的蛋白有2种(Beta-defensin 123,GARP);双源癌、单发贲门癌和单发食管癌3者相一致的蛋白有1种(sPLA2-ⅡA)。结论:所发现的基因变异位点与蛋白质组候选蛋白对比相一致的17种蛋白可能是食管和贲门癌变关键候选蛋白和基因。
OBJECTIVE: To analyze the difference of gene expression between serum protein and cancer tissue in patients with esophageal and gastric cardia double-cell carcinoma, single esophageal cancer and single cardia carcinoma, and to screen the key candidate proteins and genes of esophageal and cardiac cancer. METHODS: Four patients with esophageal and cardiac adenocarcinoma (n = 4) and single esophageal cancer (107 patients) were analyzed by SELDI-TOF-MS and comparative genomic hybridization (CGH) Single cardia cancer (86 cases) serum proteome and cancer tissue whole genome. Results: According to the consistent comparison between the candidate proteins screened by SELDI-TOF-MS proteomics and the CGH-screened genes, the proteins of the esophageal cancer serum proteome and the cancer CGH mutation loci are consistent 14 (52%, 11/21). There were two kinds of proteins (GABP, Beta-defensin 123, GARP) in gastric cardia cancer and gastric cardia cancer. Esophageal cancer 3 consistent with the protein has 1 (sPLA2-Ⅱ A). CONCLUSIONS: The 17 proteins that are found to be consistent with proteomic candidate proteins at the site of mutation are likely key candidates for esophageal and cardial carcinogenesis.