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应用 DNA 解旋荧光测定(FADU)法,研究γ射线(0.1～4.0Gy)和低剂量γ射线(0.5～8.0cGY)照射对淋巴细胞 DNA 断裂的影响,以及低剂量γ射线照射后 DNA 修复功能的变化。结果表明,FADU 法测得的0.3～4Gyγ射线所致的淋巴细胞 DNA 断裂与剂量呈线性关系(y=0.06+13.4X);0.5～8.0cGyγ射线对静止期淋巴细胞和照后丝裂原激活的淋巴细胞的双链 DNA 百分数无影响。说明低剂量照射不能减少细胞内固有(本底水平)的 DNA 断裂;低剂量辐射(2.0cGy)对15Gyγ线照射所引起的 DNA 断裂的修复(37℃,15～60min)有促进作用,但对最终修复程度(37℃,120min)无明显提高。
The effects of γ-rays (0.1-4.0Gy) and low-dose γ-rays (0.5-8.0cGY) on the DNA fragmentation of lymphocytes were studied by DNA Fluorometry (FADU) and the effect of DNA repair after low dose γ-irradiation The change. The results showed that there was a linear relationship between DNA fragmentation and dose of 0.3 ~ 4Gy γ-ray induced by FADU method (y = 0.06 + 13.4X); 0.5 ~ 8.0cGy γ-ray irradiation on resting lymphocytes and mitogen-activated The percentage of double stranded DNA in lymphocytes had no effect. The results showed that low dose irradiation could not reduce the intrinsic (background level) DNA fragmentation; low dose radiation (2.0 cGy) could promote DNA repair (15-60 min) induced by 15Gy gamma irradiation, The final degree of repair (37 ℃, 120min) no significant increase.