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目的:探讨游泳运动对攻击行为大鼠海马神经元5-HT1A-R、5-HT1B-R蛋白水平的表达变化,为运动改善大鼠攻击行为发生机制的阐明提供实验依据。方法:成年健康雄性SD大鼠36只,适应1周后随机分为:安静对照组、攻击模型组、攻击模型游泳组、入侵组。通过旷场实验检测大鼠的行为活动,采用Western-blot法及免疫组织化学法检测大鼠海马神经元5-HT1A受体、5-HT1B受体蛋白表达变化。结果:攻击模型建立成功后大鼠的总路程、平均速度、中央活动时间均较高,经过8周游泳运动干预后攻击模型大鼠的总路程、平均速度、中央活动时间均下降P<0.05;攻击模型组大鼠海马神经元5-HT1A受体、5-HT1B受体蛋白表达明显下降(P<0.05),经过8周游泳运动干预后大鼠海马5-HT1A受体、5-HT1B受体蛋白表达明显升高(P<0.05)。结论:游泳运动对攻击模型大鼠攻击行为具有改善作用,其作用机制可能与海马神经元5-HT1A受体、5-HT1B受体表达增强,使5-HT能系统功能提高,从而增强了中枢神经抑制作用有关。
Objective: To investigate the expression of 5-HT1A-R and 5-HT1B-R protein in hippocampal neurons of swimming rats after swimming, and to provide experimental basis for elucidating the mechanism of exercise-induced aggressive behavior in rats. Methods: Thirty-six adult male Sprague-Dawley rats were randomly divided into three groups: control group, challenge model group, challenge model group and invasion group. The behavioral activities of rats were detected by open-field test. The expression of 5-HT1A receptor and 5-HT1B receptor protein in hippocampal neurons were detected by Western-blot and immunohistochemistry. Results: The total distance, average speed and central activity time of rats after successful establishment of attack model were all high. The total distance, average speed and central activity time of attack model rats decreased after 8 weeks of swimming exercise intervention (P <0.05). The expression of 5-HT1A receptor and 5-HT1B receptor protein in hippocampal neurons of rats in challenge model group decreased significantly (P <0.05). After swimming for 8 weeks, 5-HT1A receptor, 5-HT1B receptor The protein expression was significantly increased (P <0.05). Conclusion: Swimming can improve the aggressive behavior of rats in challenge model. Its mechanism may be related to the increased expression of 5-HT1A receptor and 5-HT1B receptor in hippocampal neurons, which enhances the system function of 5-HT and enhances the central nervous system Neural inhibitory effect.