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生长素对果实发育起着重要的调控作用。生长素反应因子(auxin response factor,ARF)和生长素/吲哚乙酸蛋白(auxin/indoleacetic acids protein,Aux/IAA)是生长素信号转导系统中的两个关键因子,在转录水平上对生长素参与的生理活动进行调控。为探索生长素调控桃果实发育的机制,选取ARF家族的1个基因PpARF1,Aux/IAA家族的5个基因PpIAA3、PpIAA9、PpIAA17、PpIAA26和PpIAA29,克隆其全长cDNA序列并进行生物信息学分析,对它们在桃果实不同发育时期中果皮和种子的表达进行qRT-PCR检测。序列分析表明:这6个基因的编码区全长分别为2 037、594、1 194、618、384和723 bp,分别编码678、197、397、205、127和240个氨基酸;氨基酸序列比对分析显示桃PpARF1蛋白序列和草莓的FvARF1(XP_004300014.1)同源性最高,达到90.56%,PpIAA家族的5个蛋白同源性很低,只有23.77%;荧光定量PCR结果显示,在花后52 d(果实发育硬核期),PpIAA3和PpIAA17在中果皮中的表达量显著升高;PpIAA26、PpIAA29和PpARF1在种子中的表达量显著升高。预示生长素可能在桃果实发育硬核期发挥着重要的调控作用。
Auxin plays an important regulatory role in fruit development. Auxin response factor (ARF) and auxin / indoleacetic acids protein (Aux / IAA) are two key factors in auxin signal transduction system, Factors involved in the physiological activities of regulation. In order to explore the mechanism of auxin regulation of peach fruit development, five genes of PpARF1 and Aux / IAA family of ARF family were selected and cloned their full-length cDNA sequence and analyzed by bioinformatics QRT-PCR was used to detect the pericarp and seed expression in peach fruit at different developmental stages. Sequence analysis showed that the coding region of these six genes were 2037, 594, 1 194, 618, 384 and 723 bp in length, encoding 678, 197, 397, 205, 127 and 240 amino acids, respectively. Amino acid sequence alignment The results showed that the PpARF1 protein had the highest homology with that of FvARF1 (XP_004300014.1) in strawberry, reaching 90.56%. The homology of PpIAA family was very low (23.77%). Fluorescence quantitative PCR d, and the expression levels of PpIAA3 and PpIAA17 in mesocarp increased significantly. The expression levels of PpIAA26, PpIAA29 and PpARF1 in seeds were significantly increased. Indicating that auxin may play an important role in the development of peach fruit hard nuclei.