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采用高效液相色谱法测定牙鲆体内氯霉素的药物浓度,非房室模型统计矩原理分析药动学数据.结果表明:1)单次口服剂量为80 mg·kg-1的氯霉素,药物在牙鲆体内的药-时曲线呈明显双峰现象:第一次达峰时间Tmax(1)出现在2.00 h,鳃、肝、肾、血、肌的第一次达峰浓度Cmax(1)依次为15.01、11.80、10.35、8.56、5.21μg·mL-1;第二次达峰浓度Cmax(2)小于第一次的浓度Cmax(1),第二次达峰的时间Tmax(2)出现在8.00 h,药-时曲线下面积(AUC):肾、鳃、肝、血、肌分别为176.87、133.77、118.77、65.33、50.36μg·mL-1·h.消除半衰期(T1/2)为4.89-10.39 h,平均滞留时间(MRT)为8.67-17.05 h,说明氯霉素在牙鲆体内吸收较迅速,但滞留时间较长.2)连续5 d口服剂量为40 mg·kg-1的氯霉素,药物消除半衰期(T1/2)为39.40-115.50 h,说明口服氯霉素在牙鲆体内消除缓慢,残留较严重,其中以肾脏和肝脏组织中残留最明显.
The drug concentration of chloramphenicol in flounder was determined by high performance liquid chromatography (HPLC) and the pharmacokinetic data were analyzed by non-compartmental model. The results showed that: 1) chloramphenicol at a single oral dose of 80 mg · kg- The drug-time curve of drug in flounder was obviously bimodal: the first peak time Tmax (1) appeared at 2.00 h, the first peak concentration of gill, liver, kidney, blood and muscle Cmax 1) followed by 15.01,11.80,10.35,8.56,5.21μg · mL-1; the second peak concentration Cmax (2) is less than the first concentration Cmax (1), the second peak time Tmax (2 ) Appeared at 8.00 h, the area under the curve of drug-time curve (AUC) was 176.87,133.77,118.77,65.33 and 50.36μg · mL-1 · h, respectively. The elimination half-life (T1 / 2 ) Was 4.89-10.39 h, and the average retention time (MRT) was 8.67-17.05 h, indicating that chloramphenicol absorbed rapidly in flounder, but the retention time was longer.2) The oral dose of 40 mg · kg- 1 of chloramphenicol, drug elimination half-life (T1 / 2) of 39.40-115.50 h, indicating that oral chloramphenicol in flounder to eliminate slowly, residual more serious, of which the most obvious renal and liver tissue residues.