我们利用水稻苯丙氨酸解氨酶启动子(AQ630)和吲哚乙酰胺赖氨酸酯合成酶基因(iaaL)构建了维管束特异表达的植物表达载体pBAL1并导入烟草基因组中。比较了对照植株、转基因植株的幼茎和根外植体在组织培养中分化能力的变化,结果表明转基因植株茎外植体的不定芽形成明显受到促进,而转化植株根外植体在不定根发生方面对外源IAA的敏感性下降。 We constructed a vascular bundle-specific plant expression vector pBAL1 using the rice phenylalanine ammonia lyase promoter (AQ630) and the indole acetamide lysinate synthase gene (iaaL) and introduced it into the tobacco genome. Differences in differentiation between young and adult explants of control and transgenic plants were compared. The results showed that the adventitious buds of stem explants of transgenic plants were obviously promoted, but the root explants of transgenic plants were not adventitious root Sensitivity to exogenous IAA declined.