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天选43是由8845-01-01-1-1和抗源材料贵农22杂交选育而成的普通小麦品种,对我国目前所有条锈菌生理小种均表现良好抗性。为明确其抗条锈性遗传基础,本研究选用当前条锈菌流行小种CYR32和CYR33,对天选43与感病品种铭贤169杂交F1、F2和F3代群体进行遗传分析,同时应用460对SSR引物对接种CYR32的天选43/铭贤169 F2代150个单株群体进行抗病基因定位。结果表明,天选43对CYR32抗性由1对显性基因控制,而对CYR33抗性由1对隐性基因控制。筛选到10个与抗CYR32基因连锁的SSR标记Xwmc134、Xgwm413、Xbarc187、Xwmc406、Xcfd65、Xgwm18、Xbarc181、Xbarc137、Xwmc419和Xgwm230,两侧距离目的基因最近的标记为Xgwm18和Xgwm413,遗传距离分别为0.8 c M和3.4 c M,并初步将其抗病基因定位于小麦染色体1BS上,暂命名为Yr Tx43。基因来源、抗病遗传分析、分子标记检测及染色体位点分析表明,Yr Tx43很可能是与Yr24、Yr26具有等位性的抗条锈基因。
Tianzan 43 is a common wheat variety, which was bred from 8845-01-01-1-1 and Guinong22, an anti-source material, and showed good resistance to all the race species present in our country. In order to clarify the genetic basis of resistance to stripe rust, the present study selected the current race CYR32 and CYR33 races to genetic analysis of the F1, F2 and F3 generations of crosses between day 43 and the susceptible cultivar Mingxian 169, while applying 460 The SSR primers were used to locate the disease-resistant genes in 150 individuals of F2 population of Zhongnian 43 / Mingxian 169 inoculated with CYR32. The results showed that the resistance of CYR32 to CYR32 was controlled by one pair of dominant genes while the resistance to CYR33 was controlled by one pair of recessive genes. Nine SSR markers Xwmc134, Xgwm413, Xbarc187, Xwmc406, Xcfd65, Xgwm18, Xbarc181, Xbarc137, Xwmc419 and Xgwm230 linked to the anti-CYR32 gene were screened. The closest markers on both sides of the target gene were Xgwm18 and Xgwm413, respectively, and the genetic distances were 0.8 c M and 3.4 c M, and the disease resistance gene was initially mapped on wheat chromosome 1BS, tentatively named as Yr Tx43. Gene source, disease resistance genetic analysis, molecular marker detection and chromosomal location analysis indicated that Yr Tx43 is probably the stripe rust resistance allele with Yr24 and Yr26.