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目的:观察归脾丸对苯中毒小鼠低下的T细胞亚群数,以及血清溶血素和血清中粒-巨噬细胞集落刺激因子(G-CSF)水平的改善作用。方法:72只周昆明小鼠,随机分成正常对照组、模型组、西药组和归脾丸高、中、低剂量组。将正常对照组外的5组小鼠置于人工模拟高苯浓度环境下复制苯中毒模型。造模后,正常对照组、模型组给予0.9%氯化钠溶液0.2m L/只灌胃;归脾丸高、中、低剂量组分别按8、4、2mg·kg-1·d-1灌胃;西药组给予利血生1.5mg·kg-1·d-1,鳖肝醇5mg·kg-1·d-1混悬液灌胃。采血并用全自动血细胞分析仪、流式细胞仪检测外周血,T细胞亚群的计数和血清溶血素、G-CSF的水平。结果:与模型组比较,归脾丸各组与西药组的CD4+、CD8+和CD4+/CD8+的表达均显著上升(P<0.05)。归脾丸大、中剂量组能提高鸡红细胞半数溶血时的吸光度值(P<0.05);与西药组比较,归脾丸各剂量组能升高G-CSF水平(P<0.05)。结论:归脾丸对苯中毒小鼠低下的T细胞亚群CD4+/CD8+数,以及血清溶血素和G-CSF水平具有明显的改善作用。
Objective: To observe the effect of Guipi Pill on low T lymphocyte subsets and the improvement of serum hemolysin and serum granulocyte-macrophage colony stimulating factor (G-CSF) in mice with benzene poisoning. Methods: 72 Kunming mice were randomly divided into normal control group, model group, western medicine group and Guipiwan high, medium and low dose groups. Five groups of mice outside the normal control group were placed in a model of benzene poisoning replicated under artificial simulated high benzene concentration. After modeling, the normal control group and the model group were given 0.9% sodium chloride solution 0.2m L per gavage; Guipiwan high, medium and low dose groups were respectively treated with 8,4,2mg · kg-1 · d-1 Gavage; Western medicine group was given reserpine 1.5mg · kg-1 · d-1, turtle liver alcohol 5mg · kg-1 · d-1 suspension intragastrically. The blood was collected and the counts of peripheral blood and T lymphocyte subsets and the levels of serum hemolysin and G-CSF were detected by automatic hematology analyzer and flow cytometry. Results: Compared with the model group, the expression of CD4 +, CD8 + and CD4 + / CD8 + of Guipiwan group and western medicine group increased significantly (P <0.05). Guipi Wan large and medium dose group can increase the hemoglobin half hemolytic absorbance value (P <0.05); compared with western medicine group, Guipi Wan dose group can increase the level of G-CSF (P <0.05). Conclusion: Guipi Pill can significantly reduce the CD4 + / CD8 + T lymphocyte subsets and serum hemolysin and G-CSF in mice with benzene poisoning.