目的　观察血管紧张素Ⅱ (AngⅡ )对培养牛主动脉血管平滑肌细胞 (VSMC)增殖的作用及银杏内酯对其增殖的影响。方法　采用组织块贴壁法培养牛主动脉VSMC ,以3 H TdR掺入法和流式细胞仪分别测定VSMC的DNA合成量及细胞周期中各期细胞构成比。结果　AngⅡ(0 1μmol/L)作用 2 4h使3 H TdR掺入量比对照组增加 34 % ;S期和G2 /M期细胞增多 ,细胞增殖指数由 35 15 %增至 45 0 3%。与AngⅡ组相比 ,AngⅡ加银杏内酯B(G B ,0 1～ 10 μmol/L)组、AngⅡ加G B和银杏内酯A(G A)的混合物 (G AB ,0 1～ 10 μmol/L)组 的3 H TdR掺入量减少 ;S期细胞构成比和细胞增殖指数减少。结论　AngⅡ对VSMC有促增殖作用 ,可被G B和G AB所拮抗 Objective To observe the effect of angiotensin Ⅱ (Ang Ⅱ) on the proliferation of cultured aortic vascular smooth muscle cells (VSMC) and the effect of ginkgolide on its proliferation. Methods Bovine aortic VSMCs were cultured by tissue block method. The amount of DNA synthesis in VSMCs and the ratio of cells in different phases of the cell cycle were determined by 3 H TdR incorporation and flow cytometry. Results Ang Ⅱ (0 1μmol / L) for 24 hours increased the incorporation of 3 H TdR by 34% compared with the control group. The cells in S phase and G2 / M phase increased and the cell proliferation index increased from 35 15% to 45 0 3%. Compared with Ang Ⅱ group, Ang Ⅱ plus Ginkgolide B (GB, 0 1 ~ 10 μmol / L), Ang Ⅱ plus GB and Ginkgolide A (GAB, 0 1 ~ 10 μmol / L) Group 3 H TdR incorporation decreased; S phase cell ratio and decreased cell proliferation index. Conclusion Ang Ⅱ can promote the proliferation of VSMC and can be antagonized by G B and G AB