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为明确茉莉酸甲酯(methyl jasmonate,Me JA)诱导葡萄果实抗病反应的机制,以‘巨峰’葡萄悬浮细胞为试材,将其继代培养一次后分别在含10μmol·L-1 Me JA的B5培养基和含100 nmol·L-1隐地蛋白的B5培养基中(模拟病原菌接种)振荡培养15 d,每3 d测定1次细胞质量及抗病相关指标。结果显示:单一Me JA处理未激活葡萄悬浮细胞内的防卫反应;而单一隐地蛋白处理则可立即显著诱导葡萄悬浮细胞内源H2O2迸发,提升vv NPR1.1、PR1和PR2表达水平和植保素含量;经Me JA处理的葡萄悬浮细胞在添加隐地蛋白后,其细胞内出现较单一隐地蛋白处理更为显著的H2O2迸发、PR基因表达和植保素合成现象,说明经Me JA处理的悬浮细胞只在病原激发子胁迫时才表达出较强的系统抗性。因此,Me JA诱导的葡萄悬浮细胞抗病反应可归因于Priming(植物敏化过程或防御准备过程)机制。此外,经Me JA诱导的Priming反应对葡萄悬浮细胞生长无显著影响,暗示其未抑制葡萄细胞生长和胞内物质积累。
In order to clarify the mechanism of resistance induced by methyl jasmonate (Me JA) in grapevine fruits, the suspension cells of ’Jufeng’ grape were used as experimental materials and subcultured once in 10μmol·L-1 Me JA B5 medium containing 100 nmol·L-1 cryptogenetic protein (simulating pathogen inoculation) for 15 days. The cell quality and resistance-related index were measured every 3 days. The results showed that the single Me JA treatment did not activate the defensive response in grape suspension cells. However, a single cryptoprotein treatment could immediately induce the burst of endogenous H2O2 in grape suspension cells and increase the expression of vv NPR1.1, PR1 and PR2 and phytoalexin Content; Me JA-treated grape suspension cells after adding cryptoprotein, the cells appear more significant than a single cryptoprotein treatment of H2O2 burst, PR gene expression and phytoalexin synthesis, indicating that Me JA-treated suspension Cells express strong systemic resistance only when the elicitor is elicited by the elicitor. Therefore, Me JA-induced resistance of grape suspension cells can be attributed to the Priming (plant sensitization process or defense preparation process) mechanism. In addition, the MeJA-induced Priming reaction had no significant effect on grape suspension cell growth, suggesting that it did not inhibit grape cell growth and accumulation of intracellular material.