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本文报告4例着色性干皮病病人和3例正常人外周血淋巴细胞经紫外线照射和/或丝裂霉素C处理诱发SCE的观察结果。经紫外线照射的3例着色性干皮病病人淋巴细胞的诱发SCE频率比未照射的自发SCE频率均有显著增高(P<0.01),而正常人的淋巴细胞经紫外线照射诱发的SCE频率与未照射的自发SCE频率之间无显著差异(P>0.05)。经丝裂霉素C处理的2例着色性干皮病病人和1例正常人淋巴细胞的诱发SCE频率比未处理的自发SCE频率均有明显增加,但病人的增加幅度显著大于正常人(P<0.01)。结果表明,这4例着色性干皮病病人都存在着DNA切除修复功能缺陷。此外,还发现分离淋巴细胞的自发SCE频率比微量全血培养的显著地高(P<0.01)。
This article reports the results of SCE induced by UV-irradiation and / or mitomycin C treatment in 4 patients with xeroderma pigmentosum and 3 patients with normal peripheral blood lymphocytes. The SCE frequency of lymphocytes in three cases of chronic dermatitis patients exposed to UV light was significantly higher than that of non-irradiated spontaneous SCE patients (P <0.01), while the frequency of SCE induced by ultraviolet light in normal human lymphocytes was There was no significant difference in the frequency of spontaneous SCE irradiation (P> 0.05). The frequency of SCE induced by mitomycin C in 2 cases of choriocarcinoma and 1 case of normal lymphocytes were significantly higher than that of untreated spontaneous SCE, but the increase of patients was significantly greater than that of normal persons (P <0.01). The results showed that these 4 cases of patients with xeroderma pigmentosa repair exist defects in DNA repair. In addition, it was also found that the spontaneous SCE frequency of lymphocytes isolated was significantly higher (P <0.01) than the micropatch blood cultures.