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建立了同时检测水产品中6种丁香酚类麻醉剂残留量的气相色谱-质谱(GC-MS)检测方法。样品经乙腈超声提取,分散固相萃取净化后氮吹浓缩、定容,用气相色谱-质谱仪进行测定,外标法定量。6种丁香酚类化合物在1.0~200.0 μg/L的浓度范围内线性关系良好,相关系数R~2为0.9956~0.9995。又分别在罗非鱼、南美白对虾以及扇贝肌肉样品中添加1.0~10.0 μg/kg的混合标准溶液,测定平均回收率在82.2%~100.7%范围内,相对标准偏差在0.6%~7.4%之间。方法的定量限(LOQ),丁香酚、甲基丁香酚、异丁香酚、乙酰基异丁香酚为1.0 μg/kg,甲基异丁香酚和乙酸丁香酚酯为0.5 μg/kg。
A gas chromatography-mass spectrometry (GC-MS) method was developed for the simultaneous determination of six eugenol residues in aquatic products. The sample was extracted by acetonitrile, dispersed and purified by solid phase extraction, and then the nitrogen was concentrated and fixed. The sample was determined by gas chromatography-mass spectrometry and quantified by external standard method. Six kinds of eugenol compounds in the 1.0-200.0 μg / L concentration range of good linear relationship, the correlation coefficient R ~ 2 0.9956 ~ 0.9995. In addition, the mixed standard solution of 1.0-10.0 μg / kg was added to tilapia, P. vannamei and scallop muscle respectively. The average recoveries were between 82.2% and 100.7% with relative standard deviations between 0.6% and 7.4% between. The limit of quantification (LOQ) for the method was 1.0 μg / kg for eugenol, methyl eugenol, isoeugenol, and acetyl isoeugenol, 0.5 μg / kg for methyl isoeugenol and eugenol acetate.