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目的:分析肺癌A549细胞的异质性特征。方法:有限稀释法分离A549细胞的单个克隆细胞并扩大培养,对亲代与子代细胞进行生长、转移等特征的分析,了解A549细胞内部存在的异质性特征。结果:共获得6个A549细胞子细胞株,与亲代细胞比较,各子代细胞在细胞增殖能力和转移特征方面存在明显差异(P<0.05)。结论:A549细胞存在细胞增殖能力和转移特征的异质性,可能与肿瘤的发生转移相关。
Objective: To analyze the heterogeneity of lung cancer A549 cells. METHODS: Single clone cells of A549 cells were isolated and expanded by limiting dilution method. The characteristics of growth and metastasis of parental and progeny cells were analyzed to understand the heterogeneity of A549 cells. Results: Six A549 cell lines were obtained. Compared with the parental cells, the progeny cells showed significant differences in cell proliferation and metastasis (P <0.05). CONCLUSION: There is heterogeneity of cell proliferation and metastasis in A549 cells, which may be related to tumor metastasis.