利用RT PCR技术对我国广州地区急性散发性戊型肝炎病毒细胞培养分离株G93 1、G93 2、G93 3和G93 4基因组聚合酶区部分核苷酸序列 (4 52 2～ 4 76 1nt)进行检测 ,PCR阳性产物经纯化、克隆后测序。结果G93 1、G93 3和G93 4株病毒的这段序列完全相同 ,且与我国新疆暴发流行的HEV 87A株及亚洲HEV代表株的同源性为 10 0 % ;而G93 2株与它们的差异较大 ,同源性只有 79.9% ;但是 ,它与 1997年从厦门地区急性戊型肝炎病人血清中检测的X S1株同源性很高 ,达99.2 %。并对G93 2株病毒的生物学特性进行了研究 ,结果与 87A株一致。表明我国南方散发性HEV毒株的基因组存在差异 ,可能同时流行着两种不同的HEV基因型 ,但生物学性状是相同的。 The partial nucleotide sequences (4 522 ~ 4 76 1 nt) of polymerase chain exons (G93 1, G93 2, G93 3 and G93 4) of acutely sporadic HEV isolates from Guangzhou were detected by RT-PCR , PCR positive products were purified, cloned and sequenced. Results The sequences of G93 1, G93 3 and G93 4 strains were identical and showed 10 0% homology with HEV 87A strain of Asian epidemic in China and representative strain of Asian HEV. However, the differences between G93 2 strain and their strains Its homology was only 79.9%. However, it had a high homology of 99% with the strain X S1 detected in the serum of patients with acute hepatitis E in Xiamen region in 1997. The biological characteristics of G93 2 strains were also studied. The results were consistent with that of strain 87A. This indicates that there are differences in the genomes of sporadic HEV strains in southern China. Two different HEV genotypes may be prevailing simultaneously, but the biological traits are the same.