论文部分内容阅读
选用对吸胀冷害敏感的大豆(Glycine max Merrill)品种“黑河3号”为材料,从生物膜的角度,利用系列生理生化的指标论证吸湿-回干处理对大豆种子再水合过程中膜修补的关系。吸湿-回干处理可以明显提高种子抗吸胀冷害的能力,表现在种子活力的提高与种子渗漏物的减少,同时,K~+/Na~+比降低、K~+-Na~+-ATP 酶活性提高,表明在细胞质膜上的透性调控机制的运转效应得到了增强与维持。超氧化物歧化酶(SOD)活性、过氧化氢酶和过氧化物酶活性增强,挥发性醛类释放量减少,显示出细胞抗过氧化能力的增强。根据系列生理生化指标和吸水曲线的特性,作者提出了吸湿-回干诱导膜系统“预修补”的模式,确认物理与生化修补这两个修补过程的效应在“回干”后能得以保存。
Glycine max Merrill (Glycine max Merrill) variety “Heihe No.3” was selected as a material to evaluate the effect of hygroscopic-desiccation treatment on membrane repair during the rehydration of soybean seeds from the biophysical and biochemical indices relationship. The hygroscopic-desiccation treatment can significantly improve the ability of the seed to resist the imbibition and chilling injury, which is manifested in the improvement of the seed vigor and the decrease of the seed leakage, and at the same time, the K ~ + / Na ~ + ratio is decreased, while the K ~ + -Na ~ + - ATPase activity increased, indicating that in the plasma membrane permeability regulation mechanism of operational effects have been enhanced and maintained. Superoxide dismutase (SOD) activity, catalase and peroxidase activity increased, the release of volatile aldehydes decreased, showing the ability of cells to increase anti-peroxidation. According to the characteristics of physiological and biochemical indexes and water absorption curve, the author proposed a model of “pre-repair” of moisture-inducible membrane system, and confirmed that the effects of both physical and biochemical repair processes could be preserved after “drying back”.