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本实验应用连续浓度梯度聚丙烯酰胺凝胶电泳和分光光度法,在普通番茄 Lycopersiconesculentum 中,对烟草花叶病毒(TMV)五个抗性不同的栽培品种和三个半栽培型变种,在被 TMV 感染后叶片中过氧化物酶同工酶和谷氨酸脱氢酶同工酶的变化,进行酶谱分析和过氧化物酶总活性的测定。结果表明,过氧化物酶对 TMV 的感染反应十分强烈,抗性和非抗性材料都有显著的活性增加,增加的幅度与材料本身对 TMV 抗性程度有密切关系,即含有抗 TMV 基因的品种和半栽培型变种过氧化物酶活性增加的幅度,明显低于敏感型品种。由此推断,过氧化物酶有可能成为衡量番茄品种,对 TMV 抗性的一个定量化指标,在筛选抗病品种中加以应用。
In this study, continuous concentration gradient polyacrylamide gel electrophoresis and spectrophotometry were used to detect five TMV resistant cultivars and three semi-cultivars in common tomato Lycopersicon esculentum. Changes of peroxidase isozyme and glutamate dehydrogenase isoenzyme in leaves after infection were analyzed by zymogram and total peroxidase activity. The results showed that peroxidase had a very strong response to TMV infection, and both of the resistant and non-resistant materials had a significant increase in activity, which was closely related to the degree of TMV resistance of the material itself, that is, The increase of peroxidase activity in cultivars and semi-cultivars was significantly lower than that in susceptible cultivars. Therefore, it is concluded that peroxidase may be used as a quantitative indicator of resistance to TMV in tomato varieties, which is used in screening resistant varieties.