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目的建立北豆根有效部位的反相高效液相色谱鉴别和有效成分含量分析方法。方法选用Agilent C_(18)(250 mm×4.6 mm,5μm)柱,乙腈-0.05%三乙胺水溶液为流动相,梯度洗脱,流速为1.0 ml/min,检测波长为282 nm。结果13批不同来源的北豆根有效部位的HPLC指纹图谱中可检出12个相对位置稳定的色谱峰,可以此作为鉴别的指标峰,蝙蝠葛苏林碱、蝙蝠葛碱的含量分别为2.19～16.35、2.32～10.56 mg/g。结论本实验分析方法具有较强的针对性和准确性,可用于北豆根及其制剂的质量控制。
OBJECTIVE To establish an RP-HPLC method for the identification of the active fractions of Radix et. Methods The mobile phase consisted of Agilent C 18 (250 mm × 4.6 mm, 5 μm) column and acetonitrile-0.05% triethylamine in water with a gradient of 1.0 ml / min and a detection wavelength of 282 nm. Results Thirteen peaks of relative positions were detected in HPLC fingerprints of 13 active fractions from different origins of Radix et Rhizoma. The contents of daunorurine and dauricine in bats were 2.19 ~ 16.35 , 2.32 ~ 10.56 mg / g. Conclusion The experimental method has strong pertinence and accuracy and can be used for the quality control of Radix et Rhizoma Pestis and its preparations.