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为深入探讨玉米抗鞘腐病的机制,以高抗品种浚单20、9058及中抗品种郑单958、郑58为材料,测定了接种层出镰孢后苯丙氨酸解氨酶、过氧化物酶、超氧化物歧化酶、多酚氧化酶、过氧化氢酶、木质素含量及抗病相关基因PR-1、PR-2a、LOX、MPI和GAPc的表达量。结果显示:接种后不同抗性材料叶鞘部位的防御酶活性均上升,多数在6 h达到峰值,且高抗材料酶活性增幅较大;供试材料的木质素含量均上升,且高抗材料的含量高于中抗材料;PR-1、MPI、GAPc和PR-2a等抗病相关基因的表达呈上升趋势,而LOX的表达量变化趋势不明显。研究表明玉米对鞘腐病的抗性与防御酶活性、木质素含量及其抗病相关基因的表达量呈正相关。
In order to investigate the mechanism of anti-sheath rot in maize, the resistant varieties Xundan 20,9058 and the resistant varieties Zhengdan 958 and Zheng 58 were used to test the phenylalanine ammonia- Oxidase, superoxide dismutase, polyphenol oxidase, catalase, lignin content and expression level of disease-related genes PR-1, PR-2a, LOX, MPI and GAPc. The results showed that the activities of defensive enzymes in the leaf sheaths of different resistant materials increased after inoculation, most of them reached the peak at 6 h, and the activity of high-resistant enzymes increased greatly. The content of lignin in the tested materials increased, The content of disease resistance-related genes such as PR-1, MPI, GAPc and PR-2a showed an upward trend, but the trend of LOX expression was not obvious. Studies have shown that corn resistance to sheath rot and defense enzyme activity, lignin content and the expression of resistance-related genes was positively correlated.