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目的:研究新城鸡瘟病毒(NDV-L)修饰的肿瘤细胞对小鼠肝癌是否具有免疫防治作用。方法:主要采用透射电镜,免疫荧光和噻唑蓝比色法。结果:生理盐水及单纯活NDV-L对照组小鼠全部长瘤死亡,平均存活时间MST分别为16.8d和22.6d;经NDV-L修饰的瘤苗及未经NDV-L修饰的瘤苗免疫组小鼠MST分别为32.4d和19.6d。免疫治疗实验组小鼠NDV-L修饰的H22瘤苗能诱导出较强的抗肿瘤免疫效应,其抑瘤率(57％),生存率(90％),MST(44.0d)和肺转移率(20％)均比其它各组疗效明显。结论:NDV-L修饰的肿瘤细胞对小鼠肝癌具有免疫防治效应。
Objective: To study whether NDV-L modified tumor cells have immunoprotective effect on mouse liver cancer. Methods: Transmission electron microscopy, immunofluorescence and thiazolyl blue colorimetry were mainly used. RESULTS: All the tumors in normal saline and NDV-L control mice died. The average survival time was 16.8 days and 22.6 days, respectively. NDV-L modified tumor vaccines and non-NDV-L modified tumor vaccines immunized. The mouse MSTs were 32.4d and 19.6d, respectively. In the immunotherapy experimental group, mouse NDV-L modified H22 tumor vaccines can induce strong anti-tumor immune effects, with inhibition rate (57%), survival rate (90%), MST (44.0d) and lung metastasis rate. (20%) were more effective than other groups. Conclusion: NDV-L modified tumor cells have immunoprotective effect on mouse liver cancer.