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选用抗玉米丝黑穗病自交系Mo17和SH15为供体,与受体感病自交系黄早四和昌7-2构建回交群体(BC3F1BC4F2),通过田间人工接种玉米丝黑穗病原菌鉴定抗病性表现,评价群体抗病性。研究结果显示黄早四×(黄早四×Mo17)BC4F2群体发病率明显高于BC3F1群体;两个BC4F2黄早四×(黄早四×Mo17)和昌7-2×(昌7-2×SH15)群体的发病率差异较大。采用SSR标记分析抗病株的供体染色体导入片段,发现随着回交次数的增多,导入片段数量减少,但不同回交群体中供体导入片段数目明显不同。通过连锁不平衡分析,在染色体2.09和3.04区段发掘和验证2个抗玉米丝黑穗病主效QTL,连锁标记分别为umc2077和phio53或bnlg1965。本文研究结果为抗丝黑穗病基因精细定位和分子聚合育种提供了信息和材料。
Backcross population (BC3F1 BC4F2) was constructed with inbred lines Mo17 and SH15 resistant to maize head smut and Huangzao 4 and Chang 7-2 with receptor susceptible inbred lines, Smut pathogen identification of disease resistance, evaluation of population resistance. The results showed that the incidence of BC4F2 in Huangzao 4 × (Huangzao 4 × Mo17) was significantly higher than that in BC3F1 population. The two BC4F2 Huangzao 4 × (Huangzao Si × Mo17) and Chang 7-2 × (Chang 7-2 × SH15) groups the incidence of differences. The SSR markers were used to analyze the donor chromosome fragments of the resistant plants. The number of introduced fragments decreased with the increase of backcrossing times, but the number of donor introduced fragments was significantly different in different backcross populations. Through linkage disequilibrium analysis, two major QTLs for resistance to corn smut were identified and validated in chromosomes 2.09 and 3.04. The linkage markers were umc2077 and phio53 or bnlg1965, respectively. The results of this study provide information and materials for the fine mapping and molecular polymerization breeding of resistance to head smut.