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目的 :研究细胞间粘附分子 1,2 (intercellularadhesionmolecules 1, 2 ,ICAM 1,2 )在肝内胆汁淤积 (intrahepaticcholestasis ,IHC)孕鼠肝脏不同部位的表达及其意义。方法 :应用雌二醇 (ethinylestradiol ,EE)诱导孕鼠IHC ,用流式细胞仪检测肝脏ICAM 1,2的表达。结果 :(1)用药第 5天 ,孕鼠血清丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)、总胆酸 (TBA)、碱性磷酸酶 (AKP)明显升高 ,肝脏组织学检查发现IHC ;(2 )孕鼠ICAM 1,2在肝细胞、肝门静脉内皮、肝胆管上皮的表达明显增高。结论 :粘附分子 (adhesionmolecules ,AM)表达异常可能是妊娠肝内胆汁淤积 (ICP)发病机理的一个重要环节。
Objective: To investigate the expression of intercellular adhesion molecule 1,2 (ICAM 1,2) in different parts of the liver of intrahepatic cholestasis (IHC) and its significance. Methods: The IHC of pregnant rats was induced by ethinylestradiol (EE), and the expression of ICAM 1,2 in the liver was detected by flow cytometry. Results: (1) Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bile acid (TBA) and alkaline phosphatase (AKP) Study found that IHC; (2) pregnant rats ICAM 1,2 in liver cells, portal vein endothelial, hepatobiliary epithelial expression was significantly higher. Conclusion: Abnormal expression of adhesion molecules (AM) may be an important link in the pathogenesis of intrahepatic cholestasis of pregnancy (ICP).