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Objective To determine the distribution and sequence conservation of outer membrane protein X(ompX)gene in Salmonella paratyphi A isolates as well as the immunogenicity and immono-protection of ompX gene products.Methods OmpX gene in Salmonella paratyphi A isolates was detected by PCR and the amplification products were sequenced after the T-A cloning process.OmpX gene product was expressed with E.coli expression system and the expressed rOmpX was extracted by Ni-NTA affinity
Objective To determine the distribution and sequence conservation of outer membrane protein X (ompX) gene in Salmonella paratyphi A isolates as well as the immunogenicity and immono-protection of ompX gene products. Methods OmpX gene in Salmonella paratyphi A isolates was detected by PCR and the Amplification products were sequenced after the TA cloning process. OmX gene product was expressed with E. coli expression system and the expressed rOmpX was extracted by Ni-NTA affinity