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目的:利用视频跟踪系统研究舒心安神膏对斑马鱼运动行为的影响。方法:将斑马鱼浸泡在不同浓度舒心安神膏和膏方中各单味中药的养殖水中,视频跟踪系统记录给药后斑马鱼的轨迹图、运动速度、运动长度、运动时间等,同时给予光周期刺激,观察斑马鱼的运动活性。结果:光照条件下,桂枝汤组、龙骨牡蛎组、乌梅山茱萸组、熟附子、砂仁、乌梅、山茱萸可增强斑马鱼运动活性(P<0.05)。黑暗条件下,四逆汤、熟附子、山茱萸、乌梅山茱萸组斑马鱼运动活力显著高于空白对照组(P<0.05);舒心安神膏组、四逆桂枝甘草龙牡组、桂枝汤组、干姜和砂仁斑马鱼幼鱼运动活性显著降低(P<0.05)。结论:舒心安神膏对斑马鱼的运动行为有一定的抑制作用,不同组成药物对斑马鱼运动行为影响不同。
OBJECTIVE: To study the effect of Shuxin Anshen Paste on zebrafish’s motor performance by using video tracking system. Methods: The zebrafish were immersed in the aquaculture water of different herbs with different concentrations of Shuxin soothing cream and ointment. The video tracking system recorded the zebrafish’s trajectory, movement speed, exercise length, exercise time, etc. while giving the light Cycle stimulation to observe zebrafish motor activity. Results: Under the light conditions, Guizhi decoction, keel oyster group, Fructus corni, Fructus mucosae, Amomum villosum, Fructus Mori and Fructus corni could enhance the motor activity of zebrafish (P <0.05). The activities of zebrafish in Sini decoction, Fructus Corni, Fructus corni and Fructus corni were significantly higher than those in control group (P <0.05) in the darkness condition; (P <0.05). The activities of juvenile zoobenthos in ginger and Amomum villosa were significantly decreased (P <0.05). Conclusion: Shuxin Anshen cream can restrain the zebrafish’s behavior to a certain extent, and different composition drugs have different effects on zebrafish’s behavior.