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针对老式电子血球计数仪因血球重迭造成差错和计数孔堵塞造成故障等缺点,开展了激光血球计数的实验研究。实验中结合激光测量的非接触性特点,采用了液流法把血球压缩在液流中一个20微米左右的通道内,既防止了血球的重迭,又避免了阻塞现象。用我们研制的实验装置进行了红、白血球的计数线性实验;进行了重复性测量。白血球的重复精度为2%,红血球的重复精度为2.5%。对准确度的估计,因为没有标准粒子,也没有其他血球计数仪可资比较,所以采用和目测进行比较的办法。对于40组对照组红血球的测量,激光计数和目测结果用统计方法处理:相关系数为0.98:回归方程 y=(10.93x+0.35)×10~4/微升;t 值为0.12。说明激光计数和目测从统计学角度来说无差别。白血球计数情况相同。实验表明此装置还可用来鉴别粒子大小。
In view of the defects of the old-fashioned electronic hematology counter caused by the overlapping of the blood cells and the failure caused by the blocking of the counting holes, an experimental study of the laser hemacytometer has been carried out. In combination with the non-contact characteristics of laser measurement, a liquid flow method was used in the experiment to compress the blood cells in a channel of about 20 microns in the liquid flow, thereby preventing the blood cells from overlapping and avoiding the blocking phenomenon. Using our experimental device for the red and white blood cell count linear experiments; repeated measurements. White blood cell repeatability of 2%, red blood cell repeatability of 2.5%. The accuracy of the estimate, because there is no standard particles, no other blood cell counters can be compared, so the use and visual comparison approach. For the measurement of erythrocytes in 40 control groups, the laser counting and visual observation were processed statistically: the correlation coefficient was 0.98; the regression equation y = (10.93x + 0.35) × 10-4 / μl; and the t value was 0.12. This shows that laser counting and visual observation are not statistically different. White blood cell count the same situation. Experiments show that the device can also be used to identify particle size.