作者研究和阐述了曼氏血吸虫感染小鼠肝脏8周肉芽肿T细胞及20周肉芽肿T细胞的特征。将7～8周雌性CBA/J~k小鼠皮下注射25条曼氏血吸虫Puerto Rican株尾蚴。感染8周或20周后杀死小鼠取出肉芽肿肝脏,加入肝素,RPMI 1640 10u/ml,匀浆,低速离心10～15秒,沉积物中收集肉芽肿。T细胞表型特征由0.04％叠氮钠,4℃处理,0.2％IV型胶原酶建立膜标志,用免疫荧光 The authors studied and characterized the characteristics of Schistosoma mansoni infected mice liver T-cell granuloma cells of 8 weeks and 20-week granuloma T cells. Thirty-seven weeks old female CBA / J-k mice were injected subcutaneously with 25 S. mansoni Puerto Rican cercariae. Mice were killed after 8 or 20 weeks of infection and granulomatous liver was removed. Heparin was added, RPMI 1640 10u / ml, homogenized and centrifuged at low speed for 10-15 seconds to collect granulomas in the sediment. T cell phenotypic characteristics of 0.04% sodium azide, 4 ℃ treatment, 0.2% collagenase type IV membrane markers, immunofluorescence