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目的 研究乙型肝炎病毒x基因 (HBx)对肝癌细胞胰岛素样生长因子 Ⅱ (IGF Ⅱ )启动子活性的影响 ,探讨HBx基因影响肝癌恶性表型的分子机制。方法 应用定量逆转录 聚合酶链反应 (RT PCR)法检测QGY770 1肝癌细胞株和转HBx基因肝癌细胞株QGY/HBx的IGF Ⅱ成年启动子P1与胚胎启动子P3、P4的活性。结果 与QGY770 1细胞相比 ,QGY/HBx细胞IGF Ⅱ基因P1启动子活性无明显改变 (分别为 3 .12± 1.18与 3 .74± 1.87,P >0 .0 5 ) ,而P3与P4活性显著升高 (2 .2 7± 0 .68、3 .97± 1.66与 1.2 0± 0 .3 9、1.45± 0 .43比较 ,P <0 .0 5 )。结论 HBx基因可上调肝癌细胞IGF Ⅱ基因胚胎启动子P3、P4活性 ,这可能HBx基因增强肝癌恶性表型的一个重要机制
Objective To study the effect of hepatitis B virus x gene (HBx) on the activity of insulin-like growth factor II (IGF II) promoter in hepatocellular carcinoma cells, and to explore the molecular mechanism of HBx gene affecting the malignant phenotype of hepatocellular carcinoma. Methods Quantitative reverse transcription polymerase chain reaction (RT PCR) was used to detect the activity of the IGF II adult promoter P1 and embryo promoter P3 and P4 in QGY770 1 liver cancer cell line and HBx gene hepatocellular carcinoma cell line QGY/HBx. Results Compared with QGY770 1 cells, the PIG promoter activity of IGF II gene in QGY/HBx cells was not significantly changed (3.12± 1.18 and 3.74± 1.87, P > 0.05), but P3 and P4 activity Significantly increased (22.7 ± 0.68, 3.97 ± 1.66 compared to 1.2 ± 0.39, 1.45 ± 0.443, P <0.05). Conclusion HBx gene can upregulate the activity of P3 and P4 in the embryonic promoter of IGF II gene in hepatoma cells, which may be an important mechanism of HBx gene in enhancing the malignant phenotype of hepatocellular carcinoma.