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四倍体刺槐Robinia pseudoacacia是我国引种驯化历史较久的外来树种,为人工诱交植株,分速生型和饲料型。与普通刺槐相比具有更强的适生能力,对土质要求不严格,耐干旱瘠薄,在海拔2 000 m以下、降水量不低于200 mm、年平均气温不低于3℃的地区均能生长。本实验对速生型四倍体刺槐进行了组织培养及健化技术的研究,在不定芽的诱导中,以MS、1／ 2(N)MS、LS、1／2(N)LS为基本培养基,在各培养基中,以MS+BAP0．2 mg／L+NAA0．05 mg／L的组合生长情况最好,但随着继代次数的增加,其细胞分裂素要逐步降低。在生根培养中,以1／2MS+NAA0．05 mg／L+IBAO．01 mg／L生根最好。在苗木健化中,其成活率达 73．4％。
Tetraploid Robinia pseudoacacia Robinia pseudoacacia is a long history of introduction and domestication of exotic species, artificial induction plants, sub-fast-growing type and feed type. Compared with ordinary locust, it has stronger adaptability, less strict requirements on soil quality, drought-resistant barrenness, at an altitude of 2 000 m, precipitation not less than 200 mm, annual average temperature of not less than 3 ℃ in the region Grow. In this experiment, the tissue culture and the technology of quick-growing tetraploid Robinia pseudoacacia were studied in this paper. MS, 1/2 (N) MS, LS and 1/2 In each medium, the combination of MS + BAP 0.2 mg / L + NAA 0.05 mg / L was the best, but its cytokinin gradually decreased with the increase of the number of subculture. In rooting culture, 1 / 2MS + NAA0.05 mg / L + IBAO. 01 mg / L best for rooting. In seedling health, the survival rate of 73.4%.