论文部分内容阅读
目的 通过观察中枢神经系统 (CNS)中 α-微管蛋白 (α- Tub)及 β-微管蛋白 (β-Tub)的表达 ,探讨膳食锌调节 CNS微管聚合作用的可能机制。方法 80只刚怀孕 ICR母鼠随机分为严重缺锌组、轻度缺锌组、适锌组、高锌组和高锌对喂组五组 ,在孕期和哺乳期分别饲喂含 1、5、30、1 0 0和 1 0 0 mg/ kg锌水平的实验饲料 ;断奶期 (仔鼠 2 0日龄 )后各组均改喂普通饲料。取各实验组动物第 1 0天胚胎 (E1 0 )头和身体、及第 1 5天胚胎 (E1 5 )、出生第 1天 (P1 )、第 5天 (P5 )、第 1 0天(P1 0 )、第 2 0天 (P2 0 )和第 70天 (P70 ,成年 )大脑、小脑和肝组织 ,采用 Western blot技术检测其 α-Tub和 β- Tub表达情况。结果 实验仔鼠从胚胎早期 (E1 0 )至发育成熟 (P70 ) CNS中均有微管蛋白 (Tub)表达 ,而肝组织中未见有 Tub印迹出现 ;α- Tub和 β- Tub在大脑和小脑的表达模式相似 ,即发育早期有上调趋势 ,达到表达高峰 (P5～P1 0 )后表达量逐步下调 ,α- Tub在 P5达到表达高峰 ,而β- Tub在 P1 0达到表达高峰 ;比较实验组各期 Tub表达水平 ,依次为 1 mg/ kg组 <5 mg/ kg组<30 mg/ kg组 <1 0 0 mg/ kg组 ,大脑和小脑 α- Tub、β- Tub水平与膳食锌水平具有明显的依赖关系。结论 发育期锌缺乏可抑制 CNS中 α-
OBJECTIVE: To investigate the possible mechanisms by which dietary zinc regulates CNS microtubule polymerization by observing the expression of α-tubulin and β-tubal in the central nervous system (CNS). Methods Eighty pregnant ICR female rats were randomly divided into five groups: severe zinc deficient group, mild zinc deficient group, niche zinc group, high zinc group and high zinc group. Five pregnant women with ICR were fed during pregnancy and lactation respectively. , 30, 100 and 100 mg / kg of zinc respectively. After weaning (20-day-old pups), all groups were re-fed with normal diet. The day 10 (P1), the fifth day (P5), the day 0 (P1) and the body weight The brain, cerebellum and liver tissues on day 0 (P2 0) and day 70 (P70, adulthood) were examined for the expression of α-tub and β-tubin by Western blot. Results The expression of Tub was observed in the CNS of embryos from early stage (E1 0) to mature stage (P70), but not from the liver tissue. Tubulation of α-Tub and β-Tub in the brain and The expression pattern of cerebellum was similar, that is, there was a tendency of up-regulation in the early stage of development. The expression level gradually decreased after reaching the peak of expression (P5 ~ P1 0), α-Tub peaked at P5 and β-Tub peaked at P1 0. The levels of Tub expression in each group were as follows: 1 mg / kg group <5 mg / kg group <30 mg / kg group <100 mg / kg group. The levels of α- Has obvious dependencies. Conclusion Zinc deficiency during development can inhibit the expression of α-