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目的:观察戊四氮致痫大鼠脑神经型钙黏附素(N-cadherin)和神经营养因子-4(NT-4)表达的变化及灵芝孢子粉的干预治疗作用,研究癫痫的发病机制及灵芝孢子粉的作用机制。方法:健康雄性Wistar实验大鼠30只,随机分为正常对照组、癫痫模型组和灵芝孢子粉治疗组,每组10只。模型组和治疗组均用致痫亚惊厥剂量的戊四氮(PTZ)腹腔注射制作Wistar大鼠慢性点燃模型。在低温条件下迅速取脑,通过免疫组化和Westernblotting检测皮质和海马区N-cadherin和NT-4的变化。结果:实验性癫痫大鼠模型制作成功,灵芝孢子粉组和癫痫模型组实验动物均达到点燃标准,与癫痫模型组动物相比,灵芝孢子粉组大鼠潜伏期明显延长,但持续时间无显著差异。免疫组化和Western blotting检测实验结果表明:癫痫模型组实验大鼠脑海马和皮质N-cadherin含量比正常对照组增高(P<0.01);灵芝孢子粉组N-cadherins含量比癫痫模型组下降(P<0.01)。同时癫痫模型组大脑海马和皮质NT-4含量比正常对照组增加(P<0.05);灵芝孢子粉组NT-4含量比癫痫模型组增加(P<0.01)。结论:灵芝孢子粉能够降低N-cadherin的表达,调整病变神经元兴奋性,起到抗癫痫作用,还能增强NT-4的表达从而减轻癫痫发作对神经系统的损伤。
Objective: To observe the changes of neuronal cadherin (N-cadherin) and neurotrophic factor-4 (NT-4) expression in rats with pentylenetetrazole-induced epilepsy and the therapeutic effect of Ganoderma lucidum spores on the pathogenesis of epilepsy. The mechanism of Ganoderma lucidum spore powder. Methods: Thirty healthy male Wistar rats were randomly divided into normal control group, epilepsy model group and Ganoderma lucidum spore powder treatment group, with 10 rats in each group. The chronic burn model of Wistar rats was made by intraperitoneal injection of pentylenetetrazole (PTZ) in both model and treatment groups. The brain was rapidly taken at low temperature and the changes of N-cadherin and NT-4 in cortex and hippocampus were detected by immunohistochemistry and Western blotting. Results: Experimental epilepsy rat model was successfully established, and the experimental animals of Ganoderma lucidum spore powder group and epilepsy model group all reached the ignition standard. Compared with the epilepsy model group, the incubation period of Ganoderma lucidum spore powder group was significantly prolonged, but the duration was no significant difference . The results of immunohistochemistry and Western blotting showed that the content of N-cadherin in hippocampus and cortex in experimental group was higher than that in normal control group (P <0.01), while the content of N-cadherins in Ganoderma lucidum spore powder group was lower than that in epilepsy model group P <0.01). At the same time, the content of NT-4 in hippocampus and cortex in epilepsy model group was higher than that in normal control group (P <0.05). The content of NT-4 in Ganoderma lucidum spore powder group was higher than that in epilepsy model group (P <0.01). CONCLUSION: Ganoderma lucidum spore powder can reduce the expression of N-cadherin, regulate the excitability of neurons in the lesion, play antiepileptic effect, enhance the expression of NT-4, and reduce the damage to the nervous system caused by seizures.