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为研究胆汁酸是否系胆汁郁积时碱性磷酸酶活性升高的原因,作者等进行了一项在胆汁郁积开始时控制胆汁酸池内容物的动物实验。取180～250g重的雄性大鼠21只,在乙醚麻醉下,经颈静脉插入PE-10导管至上腔静脉;另一PE-10导管插入胆管,埋于皮下开口于近尾处。置大鼠于限止行动的笼子。术后禁食,但可随意饮水。引流胆汁6小时,最后10分钟的胆汁则另行收集,以测定胆汁酸的基础合成率;并由6小时引流所得的总胆汁酸量和同一时间内基础胆酸合成率之差,计算出胆汁酸池大
To investigate whether bile acids are associated with increased alkaline phosphatase activity during cholestasis, the authors performed an animal experiment to control bile acid pool contents at the onset of cholestasis. Twenty-one male rats weighing 180-250 g were anesthetized with PE-10 catheter into the superior vena cava through the jugular vein. The other PE-10 catheter was inserted into the bile duct and embedded in the subendothelium near the tail. Rats were placed in motion-restricted cages. Postoperative fasting, but free to drink water. The bile was drained for 6 hours and the last 10 minutes of bile were collected separately to determine the basal rate of bile acid synthesis. The bile acid was calculated from the difference between the total bile acid volume obtained after 6 hours of drainage and the basal bile acid synthesis rate at the same time Big pool