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采用6个不同基因型辣椒(Capsicum annuum L.)的子叶、下胚轴、去除生长点并带下胚轴和各切去一半的两片子叶、去除生长点并带下胚轴和半片子叶为外植体,分别培养在附加不同激素和AgNO3的MB5和MS培养基上,研究不同基因型、外植体、激素配比和AgNO3等对外植体不定芽诱导和芽伸长的影响。结果表明,不定芽诱导以MB5+2.0mg·L-1TDZ+1.0mg·L-1IAA+4mg·L-1AgNO3培养基最佳,诱导率最高可达100%。而芽伸长的最佳培养基为MB5+3.0mg·L-16-BA+0.5mg·L-1IAA+5.0mg·L-1AgNO3+2.0mg·L-1GA3,最高伸长率可达87.5%。生根培养基为MS+0.2mg·L-1IAA+0.1mg·L-1NAA,生根率均达100%。不同的外植体类型具有不同的不定芽诱导和芽伸长的能力,以去除生长点并带下胚轴和各切除一半的两片子叶作外植体时的诱导效果最好。用50对SSR引物检测再生植株,其中86株伏地尖的再生植株和对照植株间均没有扩增出差异条带,而茄门再生植株的SSR检测结果有12对引物表现多样性。
Cotyledons and hypocotyls of 6 different genotypes of pepper (Capsicum annuum L.) were used to remove the growing point and hypocotyls and two cotyledons cut in half to remove the growing point with hypocotyls and half cotyledons The explants were cultured on MB5 and MS medium supplemented with different hormones and AgNO3 to study the effects of different genotypes, explants, hormone ratio and AgNO3 on adventitious bud induction and shoot elongation of explants. The results showed that adventitious buds induced best with MB5 + 2.0mg · L-1TDZ + 1.0mg · L-1IAA + 4mg · L-1AgNO3 medium with the highest induction rate of 100%. The bud elongation of the best medium MB5 + 3.0mg · L-16-BA + 0.5mg · L-1IAA + 5.0mg · L-1AgNO3 + 2.0mg · L-1GA3, the maximum elongation up to 87.5% . The rooting medium was MS + 0.2mg · L-1IAA + 0.1mg · L-1NAA, rooting rate reached 100%. Different explant types have different abilities of adventitious shoot induction and shoot elongation, and the induction effect is best when removing explants with hypocotyls and cutting two cotyledons as explants. Fifty pairs of SSR primers were used to detect the regenerated plants. Among them, no differential bands were amplified between the regenerated plants of 86 plants and the control plants, while SSR test showed that there were 12 pairs of SSR markers in the regenerated plants.