本文从经典遗传学的角度分析了水稻光（温）敏核不育性，认为在定位、分离光（温）核不育基因时，应尽可能选择受１对基因控制的不育系及其分离群体。对标记基因法、同工酶标记法和ＤＮＡ标记法进行光（温）核不育基因染色体定位的优缺点及其定位结果不一致的原因进行了探讨。比较了各种植物基因克隆技术在分离光（温）核不育基因上的优缺点，认为图谱分离法虽已有一定的基础，但要在距不育基因１ｃＭ范围内找到分子标记并克隆出此基因，还需作大量的工作。ＤＤ－ＰＣＲ和ＲＤＡ技术可充分利用光（温）核不育水稻的“环境敏感型”这一重要特性，应用这两种技术可能是分离光（温）敏核不育基因的较好途径。 In this paper, we analyzed the thermo-sensitivity of rice (Oryza sativa L.) from the perspective of classical genetics. It is suggested that one pair of gene-controlled sterile lines and their Separation of groups. The advantages and disadvantages of chromosomal location of the light (warm) genic male sterility gene and its inconsistent positioning result by marker gene method, isoenzyme method and DNA labeling method were discussed. The advantages and disadvantages of various plant gene cloning techniques in isolating the light (warm) nuclear male sterile gene were compared. Although there is a certain foundation for the map segregation method, molecular markers should be found within 1 cM of the sterile gene and cloned This gene, but also for a lot of work. The DD-PCR and RDA technologies can make full use of the important characteristic of “environment-sensitive” genic male sterile rice, and the application of these two techniques may be a good way to separate the thermo-sensitive genic male sterile genes.