如何快速、微量、准确地测定蛋白质和肽的分子量,是生物化学工作者经常遇到的问题。薄层凝胶色谱技术在这方面的应用已有不少报导。虽然该技术与普通类型的薄层层析法相似,但是,由于采用了具有分子筛性质的凝胶作薄层,就使该方法在应用上有其独特之处。特别是可以用来快速测定蛋白质、肽等大分子物质的分子量。 该方法的优点是:1.简单:设备较简单,只需要一个合适的密闭箱以及玻璃板、涂布器 How to determine the molecular weight of proteins and peptides rapidly, trace and accurately is a common problem for biochemists. Thin-layer gel chromatography technology has been reported in this area. Although this technique is similar to the conventional type of thin layer chromatography, this method has its unique application due to the use of a gel-forming gel as a layer. In particular, it can be used to quickly determine the molecular weight of macromolecular substances such as proteins and peptides. The advantages of this method are: 1. Simple: The equipment is simpler and only requires a suitable closed box as well as a glass plate, applicator