论文部分内容阅读
目的:探讨 MCC、DCC 基因和 YNZ22位点杂合缺失(LOH)在原发性肝癌发生发展中的作用。方法:应用多聚酶链反应技术对35例原发性肝癌 MCC、DCC 基因和 YNZ22位点数量可变的重复序列(VNTR)区进行了分析。结果:原发性肝癌 MCC 基因杂合缺失率为25.0%;DCC 为20.0%;YNZ22位点为48.0%。若将以上位点综合分析,原发性肝癌的 LOH率则为50.0%。LOH 与临床病理参数间无相关关系。结论:MCC、DCC 基因和 YNZ22位点的 LOH 参与了原发性肝癌的发生与发展。
Objective: To investigate the role of MCC, DCC gene and loss of heterozygous YNZ22 locus (LOH) in the development of primary liver cancer. METHODS: Polymerase chain reaction (PCR) technique was used to analyze the MCC and DCC genes in 35 cases of primary hepatocellular carcinoma and variable number of repeats (VNTR) in YNZ22. Results: The heterozygous deletion rate of MCC gene in primary liver cancer was 25.0%, DCC was 20.0%, and YNZ22 locus was 48.0%. If the above sites were comprehensively analyzed, the LOH rate of primary liver cancer was 50.0%. There was no correlation between LOH and clinicopathological parameters. CONCLUSIONS: MCC, DCC genes and LOH at YNZ22 locus participate in the occurrence and development of primary liver cancer.