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目的探讨煤焦沥青烟提取物(CTPE)对人单核细胞株(THP-1)炎性因子表达的影响。方法用2μg/mL CTPE处理THP-1细胞24、48、72 h,并设立阴性对照组、二甲基亚砜(DMSO)溶剂对照组,real-time PCR检测各组细胞中白介素1β(IL-1β)、白介素8(IL-8)、肿瘤坏死因子-α(TNF-α)mRNA的表达水平,酶联免疫吸附试验(ELISA)检测各组细胞培养上清中TNF-α含量。结果染毒CTPE 24 h时,染毒组IL-1βmRNA表达水平为(2.22±0.33),均高于阴性对照组和溶剂对照组(P<0.05);染毒CTPE 72 h时,染毒组IL-8和TNF-αmRNA表达水平分别为(4.64±3.03)、(3.15±0.22),均高于阴性对照组和溶剂对照组(P<0.05);与对照组比较,染毒72 h时染毒组细胞培养上清中TNF-α含量[(538.44±53.88)pg/mL]升高,差异有统计学意义(P<0.05)。结论 CTPE刺激单核细胞后可使TNF-α、IL-1β、IL-8表达水平增加。
Objective To investigate the effect of coal tar pitch smoke extract (CTPE) on the expression of inflammatory cytokines in human monocytic cell line (THP-1). Methods THP-1 cells were treated with 2 μg / mL CTPE for 24, 48 and 72 h, and negative control group and dimethyl sulfoxide (DMSO) solvent control group were established. Real-time PCR was used to detect the expression of interleukin 1β 1β, IL-8 and TNF-α mRNA and TNF-α in each group were detected by ELISA. Results The level of IL-1βmRNA in the exposed group was (2.22 ± 0.33) at 24 h after exposure to CTPE, both of which were higher than that of the negative control group and the solvent control group (P <0.05) (4.64 ± 3.03) and (3.15 ± 0.22), respectively, were higher than those in the negative control group and the solvent control group (P <0.05). Compared with the control group, the levels of TNF- The content of TNF-αin the group of cell culture supernatant [(538.44 ± 53.88) pg / mL] increased, the difference was statistically significant (P <0.05). Conclusion CTPE can stimulate the expression of TNF-α, IL-1β and IL-8 in monocytes.