目的:建立HPLC同时测定竹节参中竹节参皂苷Ⅴ和Ⅳa的含量测定方法,并对不同产地的竹节参药材进行含量测定。方法:采用Hibar C18色谱柱,以乙腈-0.1%磷酸溶液为流动相,梯度洗脱,柱温30℃,检测波长203 nm。结果:竹节参皂苷Ⅴ在0.36~7.20μg(r>0.999)范围内线性关系良好,平均加样回收率为96.0%,(RSD=0.73%,n=6);竹节参皂苷Ⅳa在0.57~11.40μg(r>0.999)范围内线性关系良好,平均加样回收率为104.2%(RSD=0.86%,n=6)。结论:该方法结果准确、重复性好,可用于竹节参药材的质量控制。 OBJECTIVE: To establish a method for the simultaneous determination of bamboo ginseng saponin Ⅴ and Ⅳa in bamboo ginseng by HPLC and to determine the content of bamboo ginseng in different habitats. Methods: Hibar C18 column with acetonitrile-0.1% phosphoric acid as mobile phase was used for gradient elution. The column temperature was 30 ℃ and the detection wavelength was 203 nm. Results: The calibration curve of Stemonoside Ⅴ was linear in the range of 0.36-7.20μg (r> 0.999) with an average recovery of 96.0% (RSD = 0.73%, n = 6) ~ 11.40μg (r> 0.999). The average recovery was 104.2% (RSD = 0.86%, n = 6). Conclusion: The method is accurate and reproducible, and can be used for the quality control of Stemona officinalis.