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采用氯仿熏蒸法和磷脂脂肪酸(phospholipid fatty acid,PLFA)方法,分析了硫酸黏菌素(colistin sulfate,CS)残留后土壤微生物群落结构的变化.结果表明:硫酸黏菌素(wCS≥5mg·kg-1)对土壤微生物生物量碳影响显著,土壤中硫酸黏菌素浓度愈高,微生物生物量碳愈低,50 mg·kg-1的硫酸黏菌素处理使土壤微生物生物量碳下降52.1%.在整个采样周期中,每克土壤总PLFA含量在硫酸黏菌素胁迫下出现明显降低,且存在一定的剂量依赖效应.第7、49天时,低浓度组(wCS=0.5 mg·kg-1)和对照组分异不明显,第21、35天时,处理间土壤微生物群落结构多样性类型分异显著,其中高浓度组(wCS=50 mg·kg-1)与对照组分异最大.表明硫酸黏菌素可致土壤微生物群落结构多样性改变,并表现出时间差异.这可能与硫酸黏菌素在土壤中化学结构发生了改变和降解有关.
Chloroform fumigation and phospholipid fatty acid (PLFA) methods were used to analyze the changes of soil microbial community structure after the removal of colistin sulfate (CS) .The results showed that the concentration of colistin sulfate (wCS ≥5mg · kg -1) had a significant impact on soil microbial biomass carbon. The higher the concentration of sulfate colistin in the soil, the lower the microbial biomass carbon, and the soil microbial biomass carbon decreased by 52.1% at the concentration of 50 mg · kg- During the whole sampling period, the total PLFA content per gram of soil significantly decreased under the condition of colistin sulfate and there was a certain dose-dependent effect.At the 7th and 49th day, the concentration of PLCS in the low concentration group (wCS = 0.5 mg · kg -1 ) And the control group showed no obvious difference. On the 21th and 35th days, the diversity of soil microbial community structure was significantly different among the treatments, with the highest concentration (wCS = 50 mg · kg -1) Colistin sulfate may cause changes in soil microbial community structure diversity and show time differences, which may be related to the change and degradation of the chemical structure of colistin sulfate in the soil.