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目的研究乙肝病毒在乙肝患者垂直传递中的情况。方法运用分子遗传学的技术方法:单核苷酸多态性(SNP)及多聚酶链反应-单链构相多态性(PCR-SSCP),对30个家庭(68个病例)的乙肝患者及其子女进行了研究。结果在乙肝患者及其受感染后出生子女中游离型及整合型乙肝病毒持续增高,与感染前所生子女间比较差异显著(P<0.05),SNP分析结果显示在乙肝病毒基因组中,U5序列和非U5序列中许多基因位点发生了碱基替换,插入或缺失,男性乙肝患者及其受感染子女的SNP分析结果被确定。结论乙肝病毒可通过男性患者传递至子女;至此,又一个分子遗传学证据证实了了乙肝病毒的遗传性传递。
Objective To study the status of hepatitis B virus in the vertical transmission of hepatitis B patients. Methods The molecular genetic methods including single nucleotide polymorphism (SNP) and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) were used to detect HBV in 30 families (68 cases) Their children studied. Results There was a significant increase in free and integrated hepatitis B virus in hepatitis B patients and their infected children, which was significantly different from those born before infection (P <0.05). SNP analysis showed that in the hepatitis B virus genome, the sequence of U5 SNP analysis of male patients with hepatitis B and their infected children was performed with base substitutions, insertions or deletions at many of the gene loci in both non-U5 and non-U5 sequences. Conclusion Hepatitis B virus can be transmitted to children through male patients; thus, another molecular genetic evidence confirms the hereditary transmission of hepatitis B virus.