论文部分内容阅读
随着功能基因组学的发展,分子标记领域已开始从传统的随机或匿名性分子标记转向目的基因标记和功能性标记。目标起始密码子多态性(start codon targeted polymorphism,SCoT)标记是一种基于翻译起始位点的目的基因标记新技术,具有操作简单、重复性好等特点,根据ATG翻译起始位点侧翼保守序列来设计单引物,扩增产生偏向候选功能基因区显性多态性标记,适合不同层次实验室的不同领域的广泛应用,SCoT标记已开始在水稻和花生上得到运用。本文旨在介绍给研究者一种可以作为传统的RAPD、ISSR标记有效补充的目的基因标记新技术,希望其广泛的应用于生物多样性分析、遗传图谱构建、重要性状标记等方面。
With the development of functional genomics, the field of molecular markers has begun to shift from the traditional random or anonymous molecular markers to the purpose of genetic markers and functional markers. The start codon targeted polymorphism (SCoT) marker is a new technique based on the target gene of translation initiation site. It has the characteristics of simple operation and good repeatability. According to the ATG translation initiation site Flanking conserved sequences to design a single primer to amplify the dominant polymorphic marker of candidate gene regions. SCoT markers are widely used in different fields of different laboratories. SCoT markers have been used in rice and peanut. The purpose of this article is to introduce to researchers a new technique of gene marking that can be an effective complement to traditional RAPD and ISSR markers. It is hoped that it will be widely used in biodiversity analysis, genetic map construction and important trait marker.