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以大豆品种铁丰29为材料,利用开顶式气室(OTCs)研究了O3浓度升高和UV-B辐射增强单独胁迫及复合胁迫下大豆叶片抗氧化酶(SOD、POD和CAT)活性及POD同工酶谱带的变化。结果表明:O3处理大豆叶片SOD、CAT和POD活性均低于对照;UV处理下,大豆叶片SOD、CAT、POD活性也均低于对照,但基本高于O3处理;O3及UV复合处理下,加剧了SOD、CAT、POD活性的减弱。对POD同工酶研究发现,在分枝期,O3处理产生1条新谱带,开花期和结荚期均比分枝期多2条谱带,并且受胁迫处理的POD酶谱带与对照相比颜色较浅,说明在整个生育期,胁迫处理下的大豆叶片POD同工酶活性受到抑制。
To study the activities of antioxidant enzymes (SOD, POD and CAT) of soybean leaves under elevated O3 and UV-B radiation under single stress and combined stress using the soybean variety Tiefeng 29 as materials, Changes in POD isoenzyme bands. The results showed that the activities of SOD, CAT and POD in soybean leaves treated with O3 were lower than those in the control. Under UV treatment, the activities of SOD, CAT and POD in soybean leaves were also lower than those in the control, but basically higher than those in O3 treatment. Under O3 and UV treatment, Aggravated the SOD, CAT, POD activity weakened. POD isoenzyme study found that in the branching stage, O3 treatment to produce a new band, flowering and podding were two more than the branching period, and the POD zymogram band and the control phase Lighter than the color, indicating that during the whole growth period, POD isozyme activity of soybean leaves under stress was inhibited.