论文部分内容阅读
快速液体柱层析在近年来有重大进展。本文作者认为,这种方法将会很快在现代化的分析实验室中推广。本文以两个例子,简要说明用自制的低压(40大气压)液体层析仪可分离和测定药物中的有效成分。方法:在本系统中,低压泵包括一个金属筒(高61厘米,直径3.8厘米),可盛450～500毫升洗脱溶剂,能耐受50大气压的压力。泵的一端与一个压力气瓶(氦气)相连,另一端与层析柱相连。层析柱和(气瓶与泵之间的以及泵与柱之间的)导管是用特
Rapid liquid chromatography has made significant headway in recent years. The authors believe that this approach will soon be rolled out in modern analytical laboratories. In this paper, two examples are given to illustrate the separation and determination of active ingredients in pharmaceuticals using a self-made, low pressure (40 atmosphere) liquid chromatograph. Method: In this system, the low-pressure pump consists of a metal cylinder (61 cm high and 3.8 cm in diameter) to elute 450 to 500 ml of solvent and withstand a pressure of 50 atm. One end of the pump is connected to a pressure cylinder (helium) and the other end to the column. The column and tubing (between the cylinder and the pump and between the pump and the column) are special