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临床上在区别一些自身免疫性甲状腺疾病时常感缺乏简便有效的手段。本文报道用猪甲状腺组织代替手术取得的人甲状腺组织,提取其中有关的球蛋白成分,并用于检测患者血清中的自身抗体获得成功。其基本方法如下:将猪甲状腺组织捣碎,加入生理盐水浸泡,继加入硫酸铵至37％饱和度,离心,弃沉淀(杂蛋白),再加入硫酸铵至42％饱和度,高速离心,留取沉淀(甲状腺球蛋白),冷冻干燥待用。提取物于区带电泳α1-α2之间呈现单一区带。免疫电泳中与相应抗血清出现单一沉淀弧。使用鞣酸血凝法检测一部分患者和对照者血清中的甲状腺球蛋白自身抗体,初步结果为:(1)2例慢性淋巴性甲状腺炎及1例甲状腺机能减退(原因不明)和1例干燥综合征患者均为强阳性(1:40->1:1万);(2)10例甲亢患者中仅1例弱阳性(1:10);(3)63例正常人仅1例弱阳性(1:10),余均阴性。上述结果初步表明试验具有一定的特异性,与国内外报道结果相符。本法易于在一般临床实验室中推广使用。
Clinically in the distinction between some autoimmune thyroid disease often lack a simple and effective means. This paper reports the use of pig thyroid tissue instead of surgery to obtain the human thyroid tissue, extract the relevant globulin components, and for the detection of serum autoantibodies in patients with success. The basic method is as follows: the pig thyroid tissue mashed, immersed in saline, followed by the addition of ammonium sulfate to 37% saturation, centrifugation, discard the precipitate (hybrid protein), then add ammonium sulfate to 42% saturation, high speed centrifugation Take sediment (thyroglobulin), freeze-drying stand-by. Extracts showed a single band between the band electrophoresis α1-α2. Immuno-electrophoresis with the corresponding antiserum appeared single precipitation arc. Serum samples of thyroglobulin autoantibodies were detected by tannic acid hemagglutination in some patients and controls. The preliminary results were as follows: (1) 2 cases of chronic lymphatic thyroiditis and 1 case of hypothyroidism (unexplained) and 1 case of dry syndrome (1: 40-> 1: 10 000); (2) only 1 of 10 hyperthyroid patients was weakly positive (1:10); (3) only 1 of 63 normal controls was weakly positive 1:10), the remaining were negative. The above results preliminary showed that the test has a certain specificity, consistent with the results reported at home and abroad. This law is easy to promote the use of general clinical laboratories.