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目的:建立测定心神宁片中酸枣仁皂苷A和酸枣仁皂苷B含量的HPLC-ELSD方法。方法:采用高效液相色谱法。色谱柱为CAPCELL PAK MG C_(18)柱,流动相为甲醇-水(70:30),检测器为蒸发光检测器,流速为0.6 ml·min~(-1),进样量为10μl,柱温为30℃。结果:酸枣仁皂苷A进样量在0.409～4.092μg范围内与峰面积积分值呈良好的线性关系(r=0.999 9),平均加样回收率为98.7%,RSD=1.7%;酸枣仁皂苷B进样量在0.398～3.980μg范围内与峰面积积分值呈良好的线性关系(r=0.999 8),平均加样回收率为99.6%,RSD=0.8%。结论:本法简便快捷、结果准确、重复性好,可用于心神宁片的含量测定。
OBJECTIVE: To establish a HPLC-ELSD method for the determination of jujuboside A and jujuboside B in Xin Shen Ning Tablet. Methods: Using high performance liquid chromatography. The column was CAPCELL PAK MG C_ (18) with the mobile phase of methanol-water (70:30). The detector was an evaporative light detector with a flow rate of 0.6 ml · min -1. The injection volume was 10 μl, The column temperature was 30 ° C. Results: The jujuboside A injection showed a good linear relationship (r = 0.999 9) with the peak area integral value in the range of 0.409 ~ 4.092 μg with an average recovery of 98.7% and RSD of 1.7%. Jujuboside The injection volume of B was linear with the integral of peak area in the range of 0.398 ~ 3.980 μg (r = 0.999 8). The average recovery was 99.6% and the RSD was 0.8%. Conclusion: This method is simple, rapid, accurate and reproducible. It can be used for determination of Xin Shen Ning Tablet.