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兔防御素NP-1是抗性谱最广的防御素之一。为了得到转NP-1基因的小麦植株,采用电激法、基因枪法和花粉管通道法将兔防御素NP-1基因导入普通小麦品种G9501和G8901中,经过抗生素和除草剂初步筛选得到8株G9501和4株G8901的转化植株。田间抗病虫鉴定结果表明,这些植株对于白粉病、叶锈和条锈病的抗性有较大的提高,但对于蚜虫的抗性没有提高,初步认为兔防御素NP-1基因已经导入小麦,并已经在小麦中得到了表达。转基因植株的进一步检测工作正在进行中。
Rabbit defensin NP-1 is one of the most resistant defensins. In order to obtain the wheat plants transformed with the NP-1 gene, the NP-1 gene of rabbit defensin was introduced into common wheat varieties G9501 and G8901 by electroporation, particle bombardment and pollen tube pathway. Eight strains were screened by antibiotics and herbicides G9501 and 4 plants transformed with G8901. Resistance to pests and diseases in the field showed that the resistance of these plants to powdery mildew, leaf rust and stripe rust was greatly increased, but the resistance to aphids was not improved. It was initially believed that the rabbit defensin NP-1 gene had been introduced into wheat, And has been expressed in wheat. Further testing of transgenic plants is underway.