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目的了解成都市2009-2013年甲型H1N1神经氨酸酶基因变异情况。方法对2009-2013年流感样病例(ILI)咽拭子标本经病毒分离鉴定后,对毒株的NA基因进行扩增和测序,比对分析其进化特征。结果与参考毒株A/California/07/2009(H1N1)-NA比较,2009年完全变异的碱基有5个,2013年为12个。氨基酸变异也由3个发展到了6个。2013年甲型H1N1 NA的碱基和氨基酸都发生了1个回复变异。碱基和氨基酸序列的年平均变异率分别是0.86%,1.23%,经系统进化树分析,2012年和2013年形成了分枝。结论 2009-2013年成都市甲型H1N1的NA,碱基和氨基酸序列不断变异,加大了遗传距离。
Objective To understand the variation of the type A H1N1 neuraminidase gene in Chengdu from 2009 to 2013. Methods Throat swab specimens of influenza-like illness (ILI) from 2009 to 2013 were isolated and identified by virus isolation. The NA gene of the strain was amplified and sequenced, and the evolutionary characteristics were compared. Results Compared with the reference strain A / California / 07/2009 (H1N1) -NA, there were 5 completely mutated bases in 2009 and 12 in 2013. Amino acid variation from 3 to 6. In 2013, a response mutation occurred in both the base and amino acids of type A H1N1 NA. The annual average mutation rates of base and amino acid sequences were 0.86% and 1.23%, respectively. According to the phylogenetic tree analysis, the annual variation in 2012 and 2013 was formed. Conclusion The nucleotide, amino acid sequence and amino acid sequence of H1N1 in Chengdu from 2009 to 2013 are constantly changing, increasing the genetic distance.