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目的探讨多巴胺D4受体基因(DRD4)第Ⅲ外显子48bp可变数目串联重复(VNTR)多态性与暴力犯罪行为的关系。方法采用聚合酶链反应和VNTR多态性分析技术,检测重庆地区621例具有暴力犯罪人员和622志愿者DRD4第Ⅲ外显子48bpVNTR;根据DRD4第Ⅲ外显子48bp VNTR等位基因重复片段大小分为短重复等位基因(重复次数<5)和长重复等位基因(重复次数≥5)。结果 DRD4第Ⅲ外显子48bpVNTR在实验组和对照组中等位基因均以重复次数4(4Repeats,4R)和2R为主,检出等位基因最大重复次数是7R。实验组中无3.5和5.5两种等位基因,而存在6.5等位基因,而对照组却是相反的结果。DRD4 48bpVNTR等位基因重复次数在具暴力犯罪行为人群与正常人群无显著统计学差异,以18岁以下及18~30岁分组亦无统计学差异(P>0.05);长等位基因频率在暴力犯罪行为人群和正常人群没有统计学差异(P>0.05),但其长等位基因频率(4.69%)较正常人群频率大(3.15%)。结论本研究重庆地区600余份犯罪分子血样样本的DRD4第Ⅲ外显子48bp VNTR与暴力犯罪行为没有相关性。
Objective To investigate the relationship between 48 bp variable number tandem repeat (VNTR) polymorphism of exon III of dopamine D4 receptor gene (DRD4) and violent crimes. Methods Polymerase chain reaction and VNTR polymorphism were used to detect 48bpVNTR of DRD4 exon 6 in 621 violent criminals and 622 volunteers in Chongqing. According to the 48 bp VNTR allele repeat size of exon 3 of DRD4 Divided into short repeat alleles (repeat number <5) and long repeat alleles (repeat count> = 5). RESULTS: The 48 bp DNA NTR of exon 3 of DRD4 was mainly found in 4Repeats, 4R and 2R alleles in the experimental and control groups. The maximum number of alleles detected was 7R. There were no 3.5 and 5.5 alleles in the experimental group, but 6.5 alleles, whereas the control group was the opposite. There was no significant difference in the number of alleles of DRD4 48bpVNTR allele between the violent criminal population and the normal population, nor was the difference between the 18-year-old group and the 18-30-year-old group (P> 0.05) There was no significant difference between criminal population and normal population (P> 0.05), but the frequency of long allele (4.69%) was higher than that of normal population (3.15%). Conclusion In our study, more than 600 DRD4 exon 48 bp VNTRs from blood samples of over 600 criminals in Chongqing were not correlated with violent crimes.