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小鼠精子畸形试验是一种检测化学物致突变性的常用方法,具有许多优点。我们在实际工作中对其进行了一些技术上的改进,使其更加简便,并减少了实验中人为的误差,提高了准确性。常规的小鼠精子畸形试验采取精子标本的步骤是:在给药4~5周后,将小鼠处死取出双侧附睾,将其放入盛有4ml磷酸缓冲液或生理盐水的小平皿中。用眼科剪剪碎附睾,再经过滤、离心、去掉上清液用沉淀涂片。然后空气干燥、固定和染色。本文主要改进
Mouse sperm abnormality test is a commonly used method to detect chemical mutagenicity and has many advantages. We have made some technical improvements in practical work to make it more simple, reduce the human error in the experiment, and improve the accuracy. Conventional mouse sperm deformity test Sperm samples taken steps: 4 to 5 weeks after administration, the mice were sacrificed Bilateral epididymis removed, and placed in 4 ml of phosphate buffer or saline in a small dish. Cut the epididymis with ophthalmic scissors, and then filtered, centrifuged, remove the supernatant sediment smear. Then air-dried, fixed and dyed. This article mainly improves