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根据已知小麦正源基因TaDEP1 cDNA序列设计引物,成功克隆了小麦TaDEP1基因组序列,发现该基因包含5个外显子4,个内含子。通过比较该基因在六倍体普通小麦A、B、D基因组中的差异,筛选出可以区分A、B、D基因组的分子标记Ta956。以中国春缺体-四体系为材料,利用该标记将TaDEP1基因定位于小麦5A、5B和5D。进一步将Ta956扩增的基因组片段在六倍体A、B、D基因组和二倍体小麦以及四倍体小麦的同源基因组间进行比较,发现内含子区域变异较大,特别是六倍体小麦B和D基因组。进一步对TaDEP1编码蛋白分析显示TaDEP1-D的预测功能域明显区别于TaDEP1-A和TaDEP1-B,表明TaDEP1功能可能具有基因组特异性。筛选的区分不同基因组特异分子标记则为今后的品种鉴定提供了依据。
According to the known TaDEP1 cDNA sequence of wheat, we successfully cloned the TaDEP1 gene of wheat and found that the gene contains 5 exons and 4 introns. By comparing the differences in the A, B and D hexaploid wheat genomes, we screened the Ta956 molecular marker which can distinguish A, B and D genomes. Using the Chinese Spring Quarantine - Quaternary system as material, the TaDEP1 gene was mapped to wheat 5A, 5B and 5D. Further comparison of the Ta956-amplified genomic fragments between the hexaploid A, B, D genomes and the homologous genomes of diploid and tetraploid wheat revealed large variations in intronic regions, in particular hexaploid Wheat B and D genomes. Further analysis of TaDEP1-encoded proteins revealed that the predicted domains of TaDEP1-D were significantly different from those of TaDEP1-A and TaDEP1-B, suggesting that TaDEP1 function may be genomic-specific. Screening to distinguish different genome-specific molecular markers provide the basis for the future identification of varieties.