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目的探讨依达拉奉对小鼠急性脑缺血-再灌注(I-R)损伤的保护作用及机制。方法健康雄性昆明小鼠126只随机分为3组。C组18只,为假手术组;制备大脑中动脉闭塞模型,于再灌注开始后每24小时经尾静脉注射依达拉奉(Y组,54只)或等体积生理盐水(NS组,54只);分别于再灌注12、24和72 h进行行为学评分、脑组织含水量、脑梗死体积、线粒体膜电位以及蛋白质、脂质和核酸氧化应激指标的测定。结果依达拉奉能够显著降低脑I-R后行为学评分、脑组织含水量及脑梗死体积;依达拉奉可改善缺血诱导的皮质及内囊区降低的线粒体膜电位,明显降低3-硝基酪氨酸,4-羟基壬烯酸和8-羟基脱氧鸟苷的水平。结论依达拉奉通过抑制蛋白质、脂质和核酸氧化应激保护小鼠脑I-R损伤。
Objective To investigate the protective effect and mechanism of edaravone on acute cerebral ischemia-reperfusion (I-R) injury in mice. Methods 126 healthy male Kunming mice were randomly divided into three groups. 18 rats in group C were sham operation group. Middle cerebral artery occlusion model was established. Edaravone (Y group, 54 rats) or equal volume saline (NS group, 54 rats) was injected through tail vein every 24 hours after reperfusion Only). Behavioral scores, brain water content, volume of cerebral infarction, mitochondrial membrane potential, and protein, lipid and nucleic acid oxidative stress were measured at 12, 24 and 72 h after reperfusion respectively. Results Edaravone could significantly reduce the behavioral score, brain water content and volume of cerebral infarction after cerebral IR. Edaravone could reduce the mitochondrial membrane potential induced by ischemia and cortex, Tyrosine, 4-hydroxynonenoic acid and 8-hydroxydeoxyguanosine. Conclusion Edaravone protects brain I-R injury in mice by inhibiting protein, lipid and nucleic acid oxidative stress.