To obtain transgenic cabbage line with broad insect resistance,a new synthetic Bacillus thuringiensis crylBa3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained.Polymerase chain reaction(PCR)and Southern blot analyses confirmed that cry1Ba3 was successfully inserted into the genome of cabbage.Reverse transcription-polymerase chain reaction(RT-PCR)demonstrated that cry1Ba3 was expressed.Western blot results confirmed the production of insecticidal protein encoded by crylBa3.Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and CrylAc-resistant diamondback moth(DBM)larvae.